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P R O D U C T IO N O F B IO A C T IV E S U B S T A N C E S B Y U N C O N V E N T IO N A L

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TE C H N O LO G IE S

PLANTCELLGROWINGWITHPORECARRIERPENOPOLYURETHANE

G.V.Zaitseva, N.A .Konstantinova, L.V.Kozlovtseva, S.E.Strogov, V.V.Turkin

JSC "Biochimmash", Moscow, 125299

We have cultivated a choice of plant cell strains: traditional and new

strains o f P. ginseng - IFRZH-1 and DAN - 25, strain VK - 39

Lerythrorhizon

and strain ME - 75

A.euchroma.

These cells were grown in callus and

suspension cultures. The growth medium composition and other growth

conditions were analogous to those described in the normative documents.

Acco rd ing to recomendations o f investiga to rs preceded prepared

penopolyurethane was brought into the cultivation flasks before sterilization:

as cube (with 0,5 mm edge) for suspension cultures and as round bearers

(with 15 mm in thick and diameter to be equal to flasks' one) for callus

cultures. At the close of the cell cultivation cycle the yield of the wet and dry

biomass, the content o f biologically active substances (ginsenosides on

cell biomass

P.ginseng

and shikonin derivatives in cell cultures

Lerythrorhizon

and

A. euchroma)

were determined.

It has been established that penopolyurethane influence on growth and

biosynthetic activity depends on the type and strain of cells, methods of

cultivation and otther factors not controlled. Penopolyurethane does not

immobilize cells in suspension, but shikonin has not been found in cultural

fluid, whilst without penopolyurethane the shikonin content in culture medium

is nearly equal to that in biomass. Contrary to the available information there

are no reasons to assume that penopolyurethane has essential influence on

the levels o f biomass and biologically active substances in cell cultures. As

it follows from the results penopolyurethane makes possible callus cultivation

w ithout agar with the same yields of biomass and biologically active

substances. In suspension cultures o f cells - producers o f shikonin

penopolyurethane may be used for elaboration of alternative method of

shikonin separation from the cultural fluid.

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