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Table 4.

Content of flavonoids and tannins, % in Achillea crithmifolia

W. et K.

Localities

Quantity of flavonoids,

%

Quantity of tannins,

%

Pirdop - Copper melting factory

0.1218

3.5680

Sofia - Tzarigradsko shosse

0.0860

3.4290

Vitosha - Platoto

0.1213

3.4290

tannins the higher presence is found in the sample with industrial pollution

(3.568

%),

while in the other districts it is reduced (3.429

%).

According to Table 5 the highest quantity of tannins in

Tanacetum vulgare

is in industrial polluted district (8.915 %), followed by the district with background

pollution (7.315 %). The lowest quantity is in transport-polluted region (5.258

%).

The quantity of flavonoids is higher in the sample collected from transport

polluted district (0.1392

%),

followed by the region with industrial pollution

(0.1257 %), while the lowest quantity is found in background polluted district

(0.1146 %).

Table 5.

Contents of flavonoids and tannins,

%

in Tanacetum vulgare

L.

Localities

Quantity of flavonoids,

Quantity of tannins,

%

%

Pirdop - Copper melting factory

0.1257

8.9150

Sofia - Tzarigradsko shosse

0.1392

5.2580

Vitosha - Platoto

0.1146

7.3150

CONCLUSIONS

1. The main flavonoid aglycones, accumulated on the leaf surface of the

examined species

Achillea crithmifolia

are: apigenin; 6,7-dimethoxy-6-

hydroxyluteolin; 3,4'-dimethoxykaempferol; 3,6,4'-trimethoxy-6-hydroxykaemp-

ferol; and 3,6,4'-trimethoxyquercetagetin. In

Tanacetum vulgare they are\

6,4'-di-

methoxyscutellarein; luteolin; 6-methoxy-6-hydroxyluteolin; 6,3‘-dimethoxy-

6-hydroxyluteolin; 3,6-dimethoxyquercetagetin; and 3,6,3'-trimethoxyquerce­

tagetin.

2. The environmental conditions have a direct effect on the biosynthesis

of polyphenols in both species.

Acknowledgements.

The author would like to thank Senior Researcher S. Ivancheva

from Institute of Botany at Bulgarian Academy of Science in Sofia, for her assistance

to this study.

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