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MODERN METHODS OF ANALYSIS OF SILAGE MICROORGANISM:
NEW RESULTS
G. Yu. Laptev, E. A. Yildirim, L. A. Ilina
Has been applied an integrated approach for studying the composition o f microorganisms
o f plants and silage by using a modern molecular genetic methods T-RFLP, NGS-
sequencing and real-time PCR. Has been applied an the composition o f microorganisms
o f 67 samples o f cereal, bean and mixed crops forage crops at different stages o f cultiva
tion and in mature silage in five districts o f the Leningradskaya oblast. It was investigated
the content of the microorganisms at different maturation stages o f ensiling (58 samples)
with biological sourdough based bacilli and lactobacilli, and chemical preservatives. The
composition o f microorganisms o f mowed plant mass was differed sharply from the com
position o f the microorganisms o f plant grass and significantly changed during the ensil
ing by the influencing o f various factors. We found in the silage the bacteria that tradi
tionally considered typical inhabitants of the gastrointestinal tract o f mammals. That was
not allowed by the using o f classical microbiological methods. Introduction o f the strains
L. plantarum and B. subtilis in silage leads to reduction of unwanted microorganisms and
pathogens. The molds occurs on plants already at the growth stage. The quantity o f the
molds an increase in the mature silage. We found mycotoxins in all analyzed samples of
grass already at the early stages o f plant growth and silage. In the silage one mycotoxins
was increased, while other mycotoxins was decreased as a result o f activity of microor
ganisms
—
biological destructors.
Ключевые слова;
silage, T-RFLP, NGS-sequencing, real-time PCR, bacteria,
mycotoxins, molds, L. plantarum, B. subtilis.
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