ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

MONO- ANDDOUBLE POTATOTRANSFORMANTSHARBORING

FOREIGNGENESUNDERCONTROLOFTHEPATATINPROMOTOR

G.A.Romanov, T.N.Konstantinova, N.P.Aksenova, L.I.Sergeeva,

S.A.Golyanovskaya, I.A.Gukasyan, *T.Schmulling, **J.Kossmann,

**L.Willmitzer

Institute ofPlantPhysiologyRAS, 127276Moscow, Russia;

*Tuebingen University, D-72076 Tuebingen, Ger m any

**Max-P1anckInstitute forPlantMolecularPhysiology, D-14476 Golm, Germany

A series of mono- and double transformants of potato plants cv. Desiree

was produced by means of

A.tumefaciens

mediated insertion of

rolC

and

rolB

genes from

A. rhizogenes

, the yeast invertase gene and the gene for the

small subunit of the ADPG pyrophosphorylase, the latter in antisense

orientation. The invertase gene and rol genes were input under control of the

tuber specific B33 patatin promotor whereas the gene for (anti)ADPG

pyrophosphorylase was driven by a constitutive 35S CaMV promotor. Double

transformants were obtained by subsequent transformation events; the

combinations of foreign genes were rolC+invertase and rolB+(anti)ADPG

pyrophosphorylase. The presence and expression of inserted genes in

transgenic plants were confirmed by the Nothern blots and the corresponding

enzymatic activity determination. Transgenic plants were grown in sterile

culture

in vitro

in the phytotrone under different conditions and their main

morphological parameters including tuber formation were determined. As a

control untransformed Desiree plants of the same variety as well as

analogously transformed lines but harboring only marker (NPTII and GUS)

genes were used.

Plants harboring

rolC

gene alone or in combination with invertase gene

displayed most pronounced phenotypic alterations, namely deformed

elongated tubers and overall enhancement of vegetative growth. The latter

was also typical for some clones harboring rolB gene. Transgenic plants

grown on media with various sucrose content started to form tubers at lower

(rolC)

or higher (invertase) sucrose concentrations than control plants. The

suitability of these and other data obtained with

in vitro

culture for the prediction

of field parameters of transgenic potato is discussed.

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