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PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION
POSSIBILITYOFANTISENSE-RNA TECHNIQUEAPPLICATIONTO INHIBIT
OFAHM GGENE EXPRESSION IN PLANTS
V.A.Poroyko, *E.B.Rukavtsova, *Ya.I.Buryanov
PushchinoState University, Pushchino
*Branch ofInstituteofBioorganic Chemistry, Pushchino
E-Mail: ,
Hydroxymethylgluraryl-CoAreductase (HMGR, EC 1.1.1.34) regulates
the synthesis of mevalonic acid (MVA), the specific precursor of diverse
isoprenoid compounds functional in plant biochemical processes. The high
levels of sequence identity in the plant's hmg mutigene family create the
possibility o f application the antisence-RNA technique to inhibit hmg gene
expression in the wide range of plants. We have constructed the full length
hmg1 (Arabidopsis thaliana) gene and conservative domains by polymerase
chain reaction (PCR). It was shown that using of the PCR primers for the
conservative domains of hmg1 (Arabidopsis thaliana)can produce the
conservative domains hmg (Nicotiana tabacum). We made a conclusion
about availability of antisense-RNA technique for inhibition of the hmg gene
expression in plants.
The full length antisense hmg1gene was cloned into plant expression
vector pAP2034. We suppose to transformate of the different plants by using
this construction (pPR13) in order to understand effects of hmggene
expression modification.
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