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PLANT GENETIC TRANSFORMATION. CELL SELECTION AND SOMATIC HYBRIDIZATION
GENETICTRANSFORMATIONOFCOMMONWHEAT
V.A.Pukhal’skii, S.P.Smirnov, T.V.Korostyleva, E.N.Bilinskaya, A.A.Eliseeva
VavilovInstitute ofGeneralGenetics, RussianAcademy ofSciences, Moscow 117809
Russia. E-Mail:
Genetic transformation of spring common wheat (Triticum aestivum L )
was performed using A. tumefaciens. Agrobacterium strain C158„ which
cointains the cointegrate of deleted Ti plasmid pGV2260 and vector plasmid
pSIR42, was used as a vector. Plasmid pSIR42 is a vector pST6 with an
incorporated sequence under the control of promoter 35S CaMV, which
contains the inverted 0.35-kb sequence from the tetracycline resistance
gene o f plasmid pBR322. The Agrobacterium suspension was applied to
the pestils after artificial pollination in field conditions. The seed set
corresponded to 3%. The F1 plants showed no morphological differences
from the control. Dot-hybridization and PCR-analysis of genomic DNA
confirmed the existence of recombinant DNA in some plants. Repeated
transformation by the this method gave the same results in the greenhouse.
Transformation frequency was at least 2.7%. Thus, our results show that
the transformation procedure we used allows recombinant DNA to be
introduced into spring common wheat genome, which is transferred via kernels
to the next plant generations.
29S
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