ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

CONSTRUCTIONOFHERBICIDE-RESISTANTTRANSGENICPLANTS

LOTUSCORNICULATUS

A.M.Stefanowitch, S.V.Gorelova,G.N.Raldugina.

TimiryazevInstitute ofPlantPhysiologyRAN.

The capability of T-DNA agrobacterium to insert into host genome

determines a possibility to use it as vector in genetics transformation of

plants. Two clones of

Lotus corniculatus

with high regeneration ability have

been isolated in culture

in vitro.

Tile genetic transformation was produced by

method of stemps segments cocultivation with overnight culture of

Agrobacterium tumefaciens

containing plasmid

pTiBo 542

with marcer genes

res is tan t to hygromycin and kanamycin. Chymeric gene o f

phosfinotricineacetyltranspherase (bar) containing in vector determines

resistant to herbicide fosphintricine (BASTA). Green shoots were obtained

on selecting medium with 50 mg/l Km. Presence of

nptll

-gene in transformed

plants was determined by PCR-analyse. Transgenics plants phenotype is

the same as control. Rowers were fertility and produced normal seeds. Four

clones transgenics plants are resistant to BASTA in greenhouse in dose 0,4

kg/ga.

3 07

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