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ВОПРОСЫ ИСПОЛЬЗОВАНИЯ КОРМОВ
ͪКормопроизводствоͫ № 3, 2017
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23. Gaggìa F. Probiotics and prebiotics in animal feeding for safe food production / F. Gaggìa, P. Mattarelli, B. Biavati // International Journal of Food
Microbiology. — 2010. — No. 141. — Р.15–28.
24. Lacroix C. Fermentation technologies for the production of probiotics with high viability and functionality / C. Lacroix, S. Yildirim // Current
Opinion in Biotechnology. — 2007. — No. 18. — P.176–183.
References
1. Avdeenko V. N. Rynok kormovykh eubiotikov v Rossii v 2007–2009 godakh / V. N. Avdeenko // Tsenovik. — 2010. — No. 11. — P.8–9.
2. GOST 8.207-76. Gosudarstvennaya sistema obespecheniya edinstva izmereniy. Pryamye izmereniya s mnogokratnymi nablyudeniyami. Metody
obrabotki rezultatov nablyudeniy. Osnovnye polozheniya. —Moscow: Standartinform, 2006. — 7 p.
3. GOST 28178-89. Drozhzhi kormovye. Metody ispytaniy. —Moscow: Standartinform, 2007. — 51 p.
4. GOST 13496.2-91. Korma, kombikorma, kombikormovoe syre. Metod opredeleniya syroy kletchatki. —Moscow: Izd-vo standartov, 2002. — 6 p.
5. GOST 13496.3-92. Kombikorma, kombikormovoe syre. Metody opredeleniya vlagi. —Moscow: Standartinform, 2011. — 4 p.
6. GOST 13496.4-93. Korma, kombikorma, kombikormovoe syre. Metody opredeleniya soderzhaniya azota i syrogo proteina. — Moscow:
Standartinform, 2011. — 15 p.
7. Danilevskaya N.V. Farmakologicheskie aspekty primeneniya probiotikov / N.V. Danilevskaya // Veterinariya. — 2005. — No. 11. — P.6–10.
8. Emelyanova I. Z. Khimiko-tekhnologicheskiy kontrol gidroliznykh proizvodstv / I. Z. Emelyanova. — Moscow: Lesnaya promyshlennost, 1969. —
367 p.
9. Issledovatelskaya kompaniya Abercade. Proizvodstvo veterinarnykh probiotikov v Rossii otsenivaetsya v 1,5 tys. t [Elektronnyy resurs]. — Rezhim
dostupa: — data obrashcheniya: 06.11.2015.
10. Lyapustin A.V. Issledovanie protsessa glubinnogo kultivirovaniya aerobnykh mikroorganizmov: metodicheskie ukazaniya k laboratornoy rabote
№ 1 / A.V. Lyapustin. — Kirov: VyatGTU, PRIP, 1999. — 38 p.
11. Neminushchaya L. A. Tekhnologiya proizvodstva i obespechenie kachestva sinbiotikov LAKTOSUBTIL-FORTE i AVILAKT-FORTE, effektivnost ikh
primeneniya v ptitsevodstve: avtoref. diss. … d-ra biol. nauk: 03.01.06. — Shchelkovo, 2011. — 48 p.
12. Panin A. N. Probiotiki — neotemlemyy komponent ratsionalnogo kormleniya zhivotnykh / A. N. Panin, N. I. Malik // Veterinariya. — 2006. —
No. 7. — P.3–6.
13. Viryasov S. N. et al. Sposob kontaktnoy sushki mikroorganizmov: patent 2008589 Rossiyskaya Federatsiya, MPK7 F26B005/16 ot 28.02.1994.
14. Zinchenko V. B. et al. Sposob polucheniya sukhogo probioticheskogo preparata: patent 2065305 Rossiyskaya Federatsiya, MPK6 A61K35/74,
C12N1/02 ot 20.08.1996.
15. Bayguzina F. A. Sposob polucheniya tabletirovannoy formy preparata-probiotika: patent 2182009 Rossiyskaya Federatsiya, MPK7 A61K35/74,
A61K9/20, C12N1/20 ot 10.05.2002.
16. Praktikum po biokhimii: uchebnoe posobie / Pod red. S. E. Severina, G. A. Solovevoy. — 2-e izd., pererab. i dop. —Moscow: MGU, 1989. — 509 p.
17. Putova E. G. Sushka produktov mikrobiologicheskogo proizvodstva / E. G. Putova. —Moscow: Mir, 1987. — 389 p.
18. Rukovodstvo po prakticheskim zanyatiyam po mikrobiologii: uchebnoe posobie / Pod red. N. S. Egorova. — 3-e izd., pererab. i dop. — Moscow:
MGU, 1995. — 224 p.
19. Sitnikov V.V. Razrabotka probioticheskoy kormovoy dobavki na osnove molochnoy syvorotki i ee primenenie pri vyrashchivanii tsyplyat-
broylerov: avtoref. diss. … kand. biol. nauk: 03.01.07. — Saratov, 2004. — 18 p.
20. Ustyuzhaninova L.V. Opredelenie optimalnykh parametrov fermentatsii laktobakteriy shtamma Lactobacillus casei VKPM V-4990 na fugate
fermentolizata otrubey / L.V. Ustyuzhaninova, V. I. Sushkova // Sb. trudov mezhdunar. internet-konf. «Biotekhnologiya. Vzglyad v budushchee». —
Kazan: Kazanskiy universitet, 2012. — P.264–273.
21. Ustyuzhaninova L.V. Tekhnologiya proizvodstva kormovykh sinbioticheskikh produktov / L.V. Ustyuzhaninova, V. I. Sushkova // Sb. trudov I
mezhdunar. internet-konf. «Sovremennye tendentsii v selskom khozyaystve». — Kazan: Kazanskiy universitet, 2012. — P.190–196.
22. Novoe pokolenie probioticheskikh preparatov kormovogo naznacheniya / N. A. Ushakova, R.V. Nekrasov, V. G. Pravdin, L. Z. Kravtsova,
O. I. Bobrovskaya, D. S. Pavlov // Fundamental’nye issledovaniya. — 2012. — No. 1. — P.184–192.
23. Gaggìa F. Probiotics and prebiotics in animal feeding for safe food production / F. Gaggìa, P. Mattarelli, B. Biavati // International Journal of Food
Microbiology. — 2010. — No. 141. — Р.15–28.
24. Lacroix C. Fermentation technologies for the production of probiotics with high viability and functionality / C. Lacroix, S. Yildirim // Current
Opinion in Biotechnology. — 2007. — No. 18. — P.176–183.
FUTURE DEVELOPMENT OF FEED PROBIOTIC PRODUCTION
V. I. Sushkova, Dr. Biol. Sc.
L. V. Ustyuzhaninova
Institute of Biology and Biotechnology, Department of Biotechnology, Vyatka State University
610000, Russia, Kirov, Moskovskaya str., 36
E-mail:
The experiment dealt with tesƟng feed symbioƟc producƟon technology, based on grain raw materials, fermented by lactobac-
teria. Lactobacillus casei strain “VKPM V-4990”, obtained from healthy piglet gastrointesƟnal tract, exhibited probioƟc acƟvity.
Wheat middling enzyme lysate performed as a substrate in combinaƟon with urea. Tests gave 10 samples of feed symbioƟcs
for piglets and swines as liquid, concentrated, dry, concentrated immobilized and dry immobilized forms. AcƟve lactobacteri-
um concentraƟon made up over 109 CFU g-1 in all samples. SymbioƟcs were analyzed for main probioƟc and fodder characteris-
Ɵcs. All samples had high antagonisƟc acƟvity and resistance to gastric digesƟon in vitro. SymbioƟc nutriƟonal value (calculated
as absolute dry maƩer) differed slightly from that of natural wheat middlings. Values of crude protein, protein and crude fiber
were higher for concentrated and dry preparaƟon forms. Liquid and concentrated symbioƟcs, stored under 2–4°С, maintained
acƟvity for at least 7 or 3 months under 24 or 12 hours of fermentaƟon respecƟvely. Storing temperature of 20–22°С provid-
ed acƟvity of dry preparaƟons for at least 3 months. AcƟvated carbon immobilizaƟon increased Lactobacillus casei acƟvity un-
der heat dehydraƟon.
Keywords: probioƟc, feed synbioƟc, immobilized form, lactobacterium, unified technology, wheat middling enzyme lysate, an-
tagonisƟc acƟvity.
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