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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
PHENYLALANINEAMMONIA-LYASE INDUCTIONBY
METHYLJ ASMONATE IN
ARNEBIA EUCHROMA
CELL CULTURE
V V.Urmantseva, T.B.Karyagina, R.V.Chertkova, T.L.Muraviova, D.I.Bairamashvili
Institute o f Bioorganic Chemistry RAS, Moscow 117871, ul, Miklukho-Macklaya, 16/10
A rneb ia euchroma (Royle) Johnst. (Boraginaceae) ce ll culture
synthesizes red naphtoquinone pigment - shikonin with bacteriological activity.
The addition o f 100 mM methyljasmonate (MJ) in cell suspension
increased the production o f shikonin to 3-5 times (up to 1,8 g/i) and caused
growth inhibition to 35 %.
The activity of phenylalanine ammonialyase (PAL) was investigated in
order to study the role o f MJ in the stimulation o f shikonin formation. This
enzyme takes part in initial stages of shikonin biosynthesis.
The PAL activity was increased two times after one day at the addition
of MJ, remained approximately the same next two days and then decreased
to control values. The protein content in cells after 24 hours during 2 days
was 30 % higherthan the control. Polypeptides with MW 16-17,66-67,79-
94 kD were appeared in MJ-treated ce lls according to SDS-PAAG
electrophoresis. As well known PAL polypeptides MW in various cell cultures
o f higher plants varied in the range 77-83 kD.
As was shown fo r cell cultures jasmonate induced expression of specific
genes, which initiated the formation o f MJ-induced proteins, including PAL.
It is suggested that MJ activates the shikonin biosynthesis by the
regulation o f PAL formation according to the data, obtaining with the Arnebia
euchroma cell culture.
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