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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
THE EFFECTS OF SOMEMETABOLITES OF ISOPRENOIDPATHWAYON
BIOMASS PRODUCTION ANDGINSENOSIDE CONTENT IN CELL
CULTURES OF PANAXGINSENG
M .V.Khodakovskaya, V.P.Bulgakov, G.K.Chernoded, Y.N.Zhuravlev
The Institute o f Biology and SoilScience, FarEast Branch o fRussianAcademy o f
Science,
The effects of mevalonic acid (MVA), farnesol and squalene on the
biomass and ginsenoside production by cell cultures of Panax ginseng were
investigated. Mevalonates were shown to influence both parameters via more
complicated way than in was considered so far (Furuya et al., 1983; Linsefors
et al., 1989). In fact, MVA stimulated synthesis o f ginsenosides only in the
ha lf o f s ix subsequent experiments. When precursors were added in
concentrations o f 10-7to 10-3M during the early exponential growth, MVA
did not stimulate the synthesis o f the ginsenosides whereas farnesol and
squalene increased the production o f ginsenosides substantially (on 45-120
% and20-60 % than that of the control, respectively). Alternatively, ginsenoside
content was increased on 30-36 % when MVA was added in the culture
during the log phase o f growth. In those experiments, where increase in
ginsenoside content by the farnesol and squalene treatment was shown,
the quantity of precursors added in media was significantly less than increase
in ginsenoside quantity.
A ll studied substances being added into media in high doses inhibited
cell growth of Panax ginseng. Contrary, then low concentrations of MVA and
farnesol were used the growth stimulation o f the 1c culture was observed
(100% and 24%, respectively). We supposed that high levels o f exogenous
MVA, exceeded that underthe normal physiological conditions, may perturb
the normal flow o f metabolites via isoprenoid pathway.
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