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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
ABOUTQUANTITATIVEDETERMINATIONOFANALKALOID
LAPPACONITIN IN PLANT
SAMPLES OFACONITUML.
F.N.Stanikhin
Institute o fBiology Ufa Scientific CenterRAS
P lant species o f genus Aconitum L. contain a medicinal alkaloid
lappaconitin which is used for production of antiarrhythmic drug allapinin. At
present time there is research of resources and ecological investigation on
the population level of plant species of genus Aconitum L. actively carried
out and also opportunity of a cultivation of callus tissue for bioproduction o f
th is diterpene alkaloid is experimentally investigated.
A technique of determination of the contents of lappaconitin in vegetative
organs and callus tissue of Aconitum L. used by us earlier includes performing
o f extraction of lappaconitin as a salt from sample weights by diluted sulfate
acid. In further it is necessary transferthe salts of alkaloid in the form of the
basis by rising pH level of acid extract with the help o f sodium hydroxide to
final pH significance up to 9. This pH level needs to be precisely maintained,
as ju s t provided that occurs the most complete reextraction of lappaconitin
by butylacetate.
The experience of realization of a plenty of the analyses has revealed
some technical problems in achievement and maintenance of necessary
level pH by identical quantity of alkali. These difficulties are arise first, by
that acid extracts of various samples have rather significant differences in
the pH level and, accordingly, require fo r alkaiifying various quantities of
alkali. Secondly, butylacetate in alkaline condition is destroyed with releasing
out o f acetic acid, that forces also to correct the required level pH.
In the given report glycine buffer solution is offered fo r maintenance of
required pH leve! of acid extracts instead of sodium hydroxide. In case of
using glycine buffer added in equal quantities to ail samples the final pH
significance is differed from initial level on 0.05-0.10 units. The offered updating
o f the technique considerably simplifies fulfillment of a analytical procedure
and enables to increase accuracy of determination o f alkaloid.
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