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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
CULTURE
IN VITRO
OF SOME
YUCCA
SPESIES
V.M.Novikova, P.A .Karpov, V.I.Chirva
State NikitaBotanical Garden UAAS, 334267, Ukraine, Yalta
Yucca macrocarpa Engelm. and Yucca treculeana - perspective exotic
plants fo r introduction in green construction o f Black Sea coast of a UIS. As
well as other kinds of Yucca (Kintia, etc., 1987; Hostettman, Marston, 1995)
these species and their hybrid (Y. Macrocarpa x Y. Treculeana) have appeared
the capable to biosynthesis o f steroid saponins.
In culture
in vitro
the plants from seeds and embryos were grown up.
Nutrient mediums fo r generate and development o f adventitious buds and
shoots - up to 6-8 shoots in one cycle of cultivation was determined. Suriving
o f rootaged
in vitro
plants in the soil m ix achieves 70-75%. Callus was
received from ovule, ovary wall, strings o f stamens, germs, leafs and roots.
In the callus inducted from leaf and root explants the development of
morphogeny structures was observed. Was received callus with actively
differentiated on its surface rootlike structures with the well advanced zone
o f root hairs.
Studying an opportunity of reception steroid saponins by a method of
tissue culture, were analysed callus tissues of Y. macrocarpa leaf, root and
embryo o f an origin, and also callus tissue was induced from strings o f
stamens o f hybrid Y. macrocarpa x Y. treculeana. W ith the analysis were
found out only trace amount o f various steroid saponins, distinguished as is
qualitative, and quantitatively depending on primary explant and environment
of cultivation. Also trace amount of steroid saponins were founded in culture
medium. There is remarkable that saccharose, used with preparation o f
nutritious medium after some months o f cultivation was transformed
completely in fructose and was not presented even in trace amounts.
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