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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
CALLUS INDUCTION FROMACONITUMSEPTENTRIONALE
I.G.Migranova, I.A .Lukitcheva, A .G .Mardamshin*
Institute o f Biology, Ufa Science Center, RussianAcademy o fSciences, Prospect
Octyabrya 69, Ufa, Russia, 450054, E-Mail:
*Biochemistryand CytochemistryDepartment, Ufa Science Center, RussianAcademy o f
Sciences, Prospect Octyabrya 69, Ufa, Russia, 450054, E-Mail:
Aconitum septentrionale K. is a source o f alkaloid lappaconitine used
fo r antiarrhythm ic preparation allapinine manufacture. The content o f
lappaconitine in different organs o f Aconitum inconstant and depends on the
plant's age, season and place o f growing. As explants fo r callus induction
we have chosen leaves, pithy parenchyma and roots o f this herb, gathered
in the first decade o f May in Ufa’s environs. The analysis with the use o f
HPLC showed, that the content of sought product, reckoning on dry weight,
in leaves, pithy parenchyma and roots amounted to 0,03%, 0,063% and
0,482% correspondingly. Sterilization of explants been made processed by
70% alcohol during 2,5 min and by 0,2 % diacid -1,5 -25 min in consecutive
order. Roots were additionally sterilized by hydrogen peroxide during 3 min.
For callus induction we have chosen MS medium with three combinations of
growth regulators: the first - 2,4-D (2,5 mg/I) + NAA (5 mg/l), the second -
2,4-D (2,5 mg/l) + 6-BAP (10 mg/l), the third - 2,4-D (2,5 mg/l). We observed
callus formation on leaf discs in all used variants o f nutrient medium, but the
most appropriate was the third variant. Callus was formed from pithy
parenchyma on the first (optimum) and the third variants of medium. Isolation
o f callus tissue from roots was happened only on the medium with 2,4-D.
Secretion o f polyphenols didn't occur when callus was formed from different
parts o f plants, gathered on the 10th day from the beginning of vegetation. It
was difficult to get callus from older plants for plentiful isolations of polyphenols.
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