ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

CREATIONOFTRANSGENICPLANTSWITHGENESOFSEED'S

DEFENSINSFROM

AMARANTHUS CAUDATUSAND RAPHANUS

SATIVUS

A.A.Shadenkov, V.G.Lunin, S.V.Dolgov*, N.S.Lyapkova, S.S.Anisimova,

L.A.Serdobinskiy, E.V.Parashina, M.F.Shemyakin

Research Institute o fAgriculturalBiotechnologyRAAS, Moscow

*Branch o fthe Institute ofBioorganic ChemistryRAS, Pushchino

Plant defensins is a group o f relatively small (30-50 amino acids) cys-

rich peptides. They are active against a broad range o f phytopathogenic

fungi and some bacteria species. The defensins in significant concentrations

are found in seeds of cultivated plants used in the human's food. So the

ecological safety of defensins is out of doubt.

To promote the plant's resistance to fungal infections the some species

o f tobacco, horseradish, carrot, apple, potato and tomato were transformed

by defensin's genes from amarant

(Amaranthus caudatus)

and radish

(

Raphanus sativus)

under the control of 35S-promoter. The optimal variants

of cultural media were developed for regeneration and propagation of potato

sp. Desire and tomato species Beliy Naliv and Peremoga. The transgenic

origin o f tobacco and horseradish plants was successfully proved with the

help o f analyses o f the presence defensin's genes DNA and NPT-activity in

plant tissues.

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