ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

FIELDPROPERTIESOFTRANSGENICPOTATOPLANTS

L.M .Khromova,L.V.Krasheninnikowa, L.N.Kukushkina, N.A.Bashilowa,

M.N.Nikitina

All-Russia Institute o fPotato Research.

Since 1992 91 lines of Km-resistant potato plants were obtained in the

All-Russia Potato Research Inst. These plants originate from 10 different

cultivars. As a target gene these sequences included one o f the genes

listed below: 1.1,3-endoglucanase gene from anaerobic Gram-positive

bacterium

Clostridium thermocellum

; 2. pro A and pro В osm genes from

Escherichia coli;

3. human

-interferon gene 4. sequence, coding dsRNA;

5. transport gene o f tobacco mozaic virus; 6. transport gene o f transport ts

•mutant o f tobacco mozaic virus; 7. glucosoisomerase gene (xyl)

E.coli;

S .isopen ty ltran s fe ra se (T -cy t) gene from T i-p la sm id o f nos-type

A.tumefaciens; 9

.Bacillus thuringiensis

d-endotoxin gene. Target genes were

controlled by CMV 35S-promotorwith nos-terminator. All the regenerants

were rooted on kanamicin-containing medium. Later on transgenosis was

proved by: a. Southern blot-hybridization; b.NPT-test; c.PCR. A ll the lines

were further investigated in field with observance of territory isolation, careful

tuber's selection in yielding, unpermission of flowering and plant residues

destruction. It was noticed that increased resistance to such plant diseases

as late blight, ring rot and black leg were met among the transgenic plants

with high frequency. The increasing made 2-3 units. The frequency of

appearance o f plants with higher starch contents and giving richer tuber

harvest was also high in transgenic population. Different lines carrying the

same significant gene could have variable morphological changes. Plants

carrying just nptll-gene as a marker demonstrated the same properties.

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