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PLANT GENETIC TRANSFORMATION, CELL SELECTIONAND SOMATIC HYBRIDIZATION
AGROBACTERIUMTRANSFORMATION ISTHETOOLTOREVEAL
PLANT HORMONALGENES
Z.B.Pavlova, N.V.Malysheva, *L.V.Kravchenko, **V.Chmelev, L.A.Lutova
Department ofGenetics, St-PetersburgState University, 199034, Universitetskayanab.
7/9, St-Petersburg, Russia, E-Mail
*All-RussiaInstituteforAgriculturalMicrobiology (ARIAM)
**N.I. VavilovInstituteforPlantBreeding.
Pea (Pisum sativum L )genotypes with different nodule formation ability
from ARIAM collection also differ in their endogenous hormonal balance.
That was revealed by their reaction to agrobacterial transformation and
confirmed by the HPLC and ELISA analysis of main cytokinins and auxins
content.
Tumor and root formation characteristics have been analysed genetically
as the agrotransformation qualitative traits. The capacity to form roots in
response to the A. tumefaciensstrain A281 transformation was inherited as
a dominant single mendelian trait. We named gene, which is responsible for
this trait, R tf (Root forming). Genetic control of the tumor formation (A.
rhizogenes strain 8196 transformation) is probably polygenic. The results
suggest a role of revealed genes in regulation of the endogenous
phytohormonal balance, as the connection between type of the response to
the agrotransformation and the hormonal spectrum of each pea genotype
was established.
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