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PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION
INSTABILITYOFNPT II GENEEXPRESSION INTOBACCOPLANTS
CARRYINGT-DNAWITHDUPLICATIONOFB-GLUCURONIDASE
T.V.Novoselya, E.V.Deineko, M.V.Pilugin, M .L.Komarov, A.V.Kochetov,
E.A.Filipenko, V.K.Shumnyi
Institute ofCytology andGenetics ofSiberianBranch ofAcademy ofSciences, Russia,
630090, Novosibirsk-90, E-Mail:
Much attention has been paid last time to the problem of the inactivation
of the transferred genes in the plant genome. Insertion of multiple copies
may leads to loss of transgene expression. Tobacco plants were received
carrying T-DNA with npt II gene (conferring kanamycin resistance) and
duplication of b-glucuronidase gene E .coli. Stability of the transgene
expression was estimated by testing resistance of the first progeny to the
selective factor.
More than 50% of the analyzed plants exhibited instability of the npt II
gene expression connected with non-Mendelian inheritance of the transgenic
phenotype; 5,5% have a complete loss of nptll gene activity and more than
60% of the plants have in a first progeny a variegated expression of maker
gene on the level of one plant.
This investigation is supported by RFFI grant N 97-04-49334.
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