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P L A N T C E L L . O R G A N , A N D T IS S U E C O L L E C T IO N S , T H E M E T H O D S O F

G E R M P L A S M P R E S E R V A T IO N

LONG-TERMSTORINGOFSUGARBEET

IN VITRO

CULTURE

O.A.Podvigina, T.P.Zhuzhzhalova, L.A.Tsupicova

The All-Russian Research Institute o fSugarBeet & Sugar (VNIISS), Ramon, Voronezh

region

Long-term storage o f sugar beet breeding material as seed bank doesn't

solve the problem o f maintaining purity o f the gene fund completely due to

hererozygosity o f seed progeny. In this connection, development of long-

term storage technologies of plants, that are the progeny of apical and axillary

meristems, under sterile conditions, becomes quite actual. Research was

carried out in optim ization o f nutrient media composition w ith the aim to

cu lture plants-regenerants in continuous regime fo r 9 -12 months by

decreasing the growth activity. Gamborg nutrient medium (B5) with growth

inhibitor added served as a base medium. It’s determ ined, that 90-100%

survival rate after 12-month culturing was observed on media containing 0.5-

2 mg/l of ferulic and salicylic acids. And presence o f ferulic acid didn't effect

the growth and vital activity o f the explants. The ir average growth fo r one

month ranged from 1.4 to 4.2 cm, and leaves were notable fo r intensive green

co lou r and good development. Presence salicylic acid decreased the

regenerant average growth rate to 1.8-2.4 cm and significantly inhibited the

plant development. Addition o f chlorcholinchloride to nutrient medium led to

m inimum growth (from 1.0 to1.7 cm). However, the plants were greatly

inhibited, and in the period o f longterm storage, dying o f 30-100% explants

was observed.

Thus, possibility o f sugar beet regenerants culturing without transfer fo r

up to 12 months by modification o f nutrient media is shown, that offers the

prospects fo r creation and storing o f collections o f valuable sugar beet

breeding material

in vitro.

530

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