ЭлБиб

P L A N T C E L L , O R G A N , A N D T IS S U E C O L L E C T IO N S , T H E M E T H O D S O F

G E R M P L A S M P R E S E R V A T IO N

CLONALMICROPROPAGATIONINVITROOFPERENNIALPLANTSAND

LONG-TERMCOLLECTIONMAINTAINANCE

E.B.Kirichenko, *A.Coudret, *H.Sallanon, *C.Genoud-Gourichon,

T.A.Krasilnikova, G.F.Bidukova

N.V.TSITSINMain Botanical Garden, RussianAcademy o fSciences;Moscow, 127276,

Botanicheskaya ul ., 4, RUSSIA;

*Laboratoire dePhysiologie etBiotechnologies Vügüta les; 24, Avenue d esLandais,

F6311

Aubiure Cedex, FRANCE

Factors o f the optim isation o f clonal m icropropagation and long-term

maintainance o f regenerating plant were studied in detail during recent ten

years. We showed that species- and variety-specificity, age and physiological

state o f maternal plants are important for high survival ability o f unitial explants

and obtaining o f healthy plants. The influence o f phytohormones as well as

temperature, light and water regimes in the course o f shoot formation and

th e ir rooting on the multiplication coefficient was studied. Some specific

pecularities o f photosynthetic apparatus biogenesis in autotrophic and

m ixotrophic tissue culture were revealed. Nearly 30 species and varieties o f

woody (ornamental, essential oil-producing and wild roses; ananas, rubber

plant etc.) and 30 species and ecotypes o f herbaceous (mentha, lavanda,

lavandin etc.) plants were maintained

in vitro

during many years. These

plants preserved their potential capacities fo r acclimatisation and cultivation

under field conditions.

5 2 6

Научная электронная библиотека ЦНСХБ