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P L A N T C E L L , O R G A N , A N D T IS S U E C O L L E C T IO N S , T H E M E T H O D S O F
G E R M P L A S M P R E S E R V A T IO N
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WAYSOFCREATIONCOLLECTIONGRAPEVINEGENETICSTOCK
(GENOFUND)
N.P.Doroshenko, G.V.Sokolova.
Russian Viticulturaland Wine-makingResearch Institute named afterIa. Potapenko.
The ways o f prolonged grapevine storage
in vitro
bymeans o f growth
m inim ising test-tube plants were investigated: prolonged storage under
positive lowering temperatures, applying CCC, introducing mannitoi and
sorbitol, 6-BAP-into culture medium and natural growth inhibitors. Positive
lowering temperature (10,5-12,0°C) and lighthess (5 0 0 l) promotes growth
lim ita tion o f te st-tub lep lan ts and more prolonged cu ltiva tion w ithout
transplanting. New storage conditions o f collection grapevine genofond
in
vitro
w ithout transplanting during 12 months and more by means o f applying
specisi medium and t 4-5°C were developed in cooperation with the laboratory
o f criostorage o f T im iryazev’s Institute o f plant Physiology. Increasing
prolonged plant storage up to 4 months applying 60 mg/l sorbitol into culture
medium , up to 6 months applying I mg/l CCC were proved to be possible.
Introducing of 0,1 mg/l BAP into culture medium and increasing saccharase
content up to 6% influenced the dem inishing root length o f test-tube plants
more then 6 tim es and shoot lihgth 3,5 times. This achieved growth
m inim isation can be used fo r more prolonged storage o f grapevine genetic
fund. Applying milled grapevine seeds in the amount o f 1,0-2,0% o f medium
volume gives such level of, natural inhibitors (abscisin acid, phenols, and so
on) at which root formation decreases in 1,7 times and root growth in 3,6,
shoot length in 3,5-5,7, their mass deminishes in 3-5 times node number on
the shoot more then in four times. As a sequence the periods between plant
fransplantation into fresh culture medium increases in 3-5 times that can be
done in 5-8 months instead o f 1,5-2 month. It rises the economic effectiveness
o f storage, as labour expanses decreases and growth inhibition of test-tube
plants is reached w ithout additional wastes o f labour and time.
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