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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
GROWTHAND PHOTOSYNTHETIC APPARATUS OFSAINTPAULIA
(SAINTPAULIAIONANTHA H.WENDL), OBTAINED BY PROPAGATION IN
VIVO AND IN VITRO
S.Tischenko, R.Karnachuk
D epartm en t o f P lan t Ph ysiology and B iotechnology, Tomsk State University, 36
Lenina st., Tomsk 634050 Russia. E -M ail;
Photosynthetic function to a large extent depends on processes of
growth and development o f a plant. Using cell and tissue cultures to study
photosynthesis processes w ill allow to find out if the plants, obtained via in
vitro method, are able to form a normal photosynthetic apparatus (PA), and
how it is reflected on the further life of the plant, grown under in vivo conditions.
T he re was m ade a com pa rison o f grow th ch a ra c te ris tic s and PA
characteristics, taken fo rtwo kinds of Saintpaulia - Festivalnaya and Magia,
bred by traditional propagation from leaf stalks and microclonal propagation
on MS medium w ith optim ized concentration o f phytohormons. The
observations were made during the process o f adaptation of the plant to its
independent growth under in vivo conditions. Plants o f kinds Festivalnaya
and Magia d iffer from each other by intensivity of growth processes and by a
number o f structural and functional organization characteristics of PA, such
as the number o f chloroplasts per cell, pigment concentration per square
un it and per ch loroplast, pho toregene ra tive ac tiv ity o f chloroplasts.
Micropropagation of Saintpaulia in vitro has intensified the growth processes
o f plants during their further cultivation in vivo, and this intensification has
been indicated by increasing o f dry biomass of the plant and its leaves, as
well as by increasing o f a number of tiers grown during the same period o f
time. While micropropagation Saintpaulia in vitro, the number o f chloroplasts
per cell and the synthesis o f pigments per square unit and per chloroplast
increase, the correlation between chlorophyll changes. There was shown
the changes of these characteristics for different kinds of plants. There was
found out the interrelation between pigment concentration and growth indexes,
as well as negative correlation between proportions of chlorophyll a and
chlorophyll b and growth indexes. W ith the transition from traditional
propagation method to micropropagation in vitro an amplification of interrelation
between pigment concentrations per square unit of leaf is observed. Factor
analysis has shown that propagation of Saintpaulia in vitro above all influences
the growth processes, its influence on structural and functional indexes of
PA is secondary.
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