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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T iO N

GRAPEVINEMERISTEMASEPTICCULTURE

O.S.Tishenco, *A.B.Burgutin.

TranscarpatianAgri-Industrial Reseach Institute,Bacta, Berestovsky region, 295520, the

Ukraine.

* Timiriazev Plant Physiology Institute, RSA, 35 Botanical str., 127276, Russia. E-Mail:

The subjects of our investigation are grapevine cultivars and breeding

lines such as cv.Russky ranny, Golubock, Smuglianka moldavskaya, Crimea

pearl, Venoslevy, Lesseya, V-95-3, X1-37-32, X1-38-92from Transcarpatian

Agri-Industrial Reseach Institute nurcery. The apical meristems were dissected

from : a) the apical and axillary buds of actively growing field shoots; b) the

green shoots initiated from cuttings stokced during the Autumn-Winter period.

A carefull dissection enabled to isolate apices from shoot tips and leaf axils

of juvenile parts of vine without surface sterilization. About 100 meristems of

200-1500 mcm size had been dissected. W ithin two first weeks nearly 97%

in c u b a tin g m e ris tem s dem on s tra te d the v ig o ro u s d iffe re n tia tio n .

Contamination was found out only in 2% initial explants. Only two meristems

from twelve ones o f 200 to 400 mcm size turned out to be lacking vital

capacity. While the rest ten exhibited the same growth rate as the larger

ones. Any visible differences in apices of the various taxons was not revealed

when growing them in the medium supplemented by cytokinin BA-6. The

explants linear sizes increased in 20-40 times by the 30-th day o f

in vitro

cultivation. The m inimum fresh weight o f the explants passed into the

hormone-free medium was 128mg , and the maximum one was 819mg.

Later on the shoots initiated from the obtained apical structures were

micropropagated. These facts evidence the possibility to dissect successfully

vine apical meristems w ithout using any sterilizing agents.

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