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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
CLONALMICROPROPAGATION OF PLANTS FROM GENUS
AGLAONEMA SCHOTT.
T.M. Cherevchenko, A.N.Lavrentyeva
M.M. Grishko Central Botanical Garden, NationalAcademy o fSciences o f Ukraine.
Timiryazevska St. 1, Kyiv - 252014 Ukraine. E mail: cherevchenko @ botanical -
T ropical and subtropical species o f Aglaonema Schott. are very
ornamental and characterized by high resistance to unfavourable condition
ofinteriors, thefore they are widely used in get planting material
we exemined plant micropropagation of genus Aglaonema: A. ’Silver Qeen’,
Acommutatum , A. commutatum ’Pseudobracteatum’, A. marantifolia, A.
treubii. For propagation we selected young shoots of 10cm in length. Explants
were segments of leaves, apical and lateral buds o f young shoots. Sterile
explants were got by sterilization with 20% and 5% Clorox and ampicillin, in
consequentive order. Studing morphogenetic potentions of explants it was
determ ined, that using of segments is unadvantaeous, becouse of it was
not success to get any structures from such segments. Regenerative ability
o f axillary buds, used as explants depended on the ir disposition on shoot.
Buds o f basal part of shoot has high morphogenetic potential and buds from
apical partvery low. We demonstrated, that morphogenetic ability of buds is
directly proportional to capacity. The more components are is bud, the higher
is its regenerative potential. Common fo r all isolated cultures of aglaonemas
is very slow growth and number of phenolic compounds, evolved in nutrient
medium. Optimal growth and development of aglaonemas cultures were found
on nutrient medium M-S with application o f 80 mg/l adenin, 3 mg/l NAA and
2 g/l activated charcoal. In some casesto increase explants growth is possible
by changing conditions of cultivation, so decreasing photoperiod from 16 to
10 h. and increasing temperature of cultivation from 26 °C to 30°C promote
successful growth o f all aglaonemas isolated cultures. Obtained plants-
regenerants were planted in substrate consists: of sphagnous moss and
sand (1:1).
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