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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

IMPROVEMENTAND CLONALMICROPROPAGATION OFYACON

G.B.Tjukavin

All-Russian Research Institut o f Vegetable Breeding and SeedProduction, Odincovo,

Russia, E-mail:vniissok@

cea.ru

Yacon

(Polymnia sonchifolia Poepp. & Endl.)

- beautiful, compact,

perennial grassy plant of height 2 - 2,5 meters growth on the eastern slopes

o f the Andes. It can be used as technical, fodder and vegetable culture. The

special interest represent reserving tybers which have been described as

being like a freshpicked apples w ith mild, sweet flavor rem iniscent of

watermelon.. Fresh tubers contain about 20 %, and dry more than 60 % of

sugars, which basically consist from inulin. Yacon introduced in USA New

Zealand and Western Europe. In Russia the work on introduction o f yacon

was begun by the research workers VNIISSOK (Kononkov, Tjukavin, 1995).

Explants fo r clonal micropropagation of yacon served apex and swelled

buds, and also fragments o f stalks young plantlets received from gemmae

of rhizome. Under cultivation of stalk explannts on MS medium with 2,4-D (2

mg/l) were received calluses, howeve rto receive regenerated-plantsfrom

them it was not possible. During of researches it was revealed latent bacteritic

affect of planntlets. Therefore fo r reception free from an infection regenerated-

plants from buds the complex sterilization them with subsequent cultivation

on media with an antibiotic was used (Tjukavin, 1995).

Apex and swelled buds of yacon were cultured on MS medium as without

hormones as supplemented with gibberellin and kinetin. Was established,

that the buds o f yacon are hormones independent. The essential importance

has a condition o f medium .The best results were received on liquid MS

medium w ithout hormones. In this case more intensive development both

plantlets and root system were observed.

Improved and propagated

in vitro

the regenerated-plants of yacon were

transplanted - in peat pots with m ixture of soil and adapted fo r conditions

vivo

with the raised humidity during four weeks. Further they well developed

in usual conditions. In a spring-summer's period the regenerated-plants of

yacon were grown in conditions o f the protected and opened ground where

the further study them organogenesis was carried out.

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