ЭлБиб

HEALTHY PLANTS AND MICROPROPAGATION

EFFECTO FPACLOBUTRAZOLONTHEPROPAGATIONO FGLABIOLUS

IN VITRO

G.I.Sedov

Transnisrian Institute o fAgriculture, RepublicaMoldova, Tiraspol, st. Mira, 50.

G ladiolus are apopular cutflower crop grown the world over. W ith

increasing demands o f the horticultural industry fo r higbquality.diesease-

?ree propagation stock, tissue culture technigues w ill become more and

more essential along the production line. According to our investigation the

padobutazol (РАС) induced the intensive proliferation ofbuds and inhibited

the lea f growth in the nutrient: medium with 6 - benzylam inopurme (BAP)

and naphthalenacetic acid (NAA) .The highest number o f shoots per explant

(the apical buds) have been obtained in the presense o f lmg/1 РАС. The

presence o f РАС (I0mg/l) in the medium with high level sucrose (60g/l) incresed

diameter ofconniets and reduced period formation conniets in 1,5 times.Shoots

subculfured to liquid medium containing РАС 0,1 mg/l and IAA 0,5 mg/l

(indolylacetic acid) fonned short roots and leaf with thick cuticula. These

shoots transplanted in the fild directly without adaptation in wet

our laboratory the two efficient systems of mass propagation o f the gladiolus

plants have been alaborafed. The first sistem o f mass propagation of the

gladiolus plants destined fo r countries w ith cold season.This sistem permit

to obtain the gladiolus minicomilete in vtro during all the year. The minicormlets

keep by low temperature (-+4 +8°C) ve ry well. The m inicorm lets are

accumulated fo r planting in the fild . TTIis sistem was approbated in Moldova.

The second sistem of mass propagation of the gladiolus plants destined for

countries w ithout cold season.This sistem perm it to obtain rooting plants

without preliminary adoptation to low humidity air. The sistem was approbated

in Colombia.

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