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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
STEVIA REBAUDIANA
MICROPROPAGATIONGENETICSTABILITY
PLANTINGMATERIAL
A.E.Rybalko,L.G.Kharuta
Scientific center ofMeristem Cultures, 354354, Sochi, Russia, tel/fax (862-2)-44 71 92
Last years interest to natural sugar substitutes production is growing.
The usage o f
Stevia rebaudiana
as stevioside natural producer is o f great
interest. Methods o f raw material production in terms of different ways o f
these planting material growing are under development. It’s proved vegetative
propagation results in more stable content and fraction composition of the
final product.
Our research and production facilities allows us to start growing
Stevia
rebavdiana
planting materials for producing the above mentioned raw materials
in subtropical of Northern Caucasus. For planting materials growing the clonal
m icropropagation method was used. This method protected by the patent
USSR № 1608845 o f22.07.1990was in operation at our enterprise fo r many
years fo r wide range o f plants microcloning. Usually it took 4 weeks to get 5-
8 intemodes plant. Growthregulators influence to
Stevia rebaudiana
callus
development in Murashige-Scoog media was tested. As per research results
the Stevia rebavdiana callus grew most intensively in media IAA, BAP of 1
mg/l.
Method o f plant acclimatization and growing was developed as well as
method o f Stevia rebavdiana propagation by cuttings. Periit with addition o f
NAA solution by 30 mg/l used fo r rooting o f the cuttings. If took 12-15 days
to get the cutting rooted. In w intertime we studied the possibility f the plants
surviving in green house with no heating. The biggest part of the plants were
alive down to-6°C .
Thus the methods of
Stevia rebavdiana
plants micro cutting, callus
producing, plant industrial propagation by green cuttings were developed. If
g ives us on opportunity to start the raw material fo r steviosid industrial
production.
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