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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

EFFECTOF IMMUNOPROTECTORSANDVIRUSINFECTIONON

PHYTOLECTINACTIVITYINPOTATO

VITRO

N.A.Rozhnova, G.A.Gerashchenkov, S.Y.Gyljazetdinov, *A.V.Babosha

Department o fBiochemistry and Cytochemistry, Ufa Scientific Center, Russian Academy

o f Sciences, 69,prospect Oktyabra, Ufa, 450054, Russia. E-Mail:

*Institute o fpotatofarming, Russian Acadamy o fAgricultural Sciences, Korenevo,

Moscow oblast', Russia

Lectins alongside with the other carbohydrate-binding proteins attract

attention of the plant immunity specialists in connection with their involvement

in many processes o f biorecognition, which are important fo r cells and

organisms. These studies are based on the lectin ability to recognize and

bind specifically the sites o f complex carbohydrates without its modifications.

The last circumstance has stipulated the interest in searching a role o f

these proteins in plant pathogenesis and resistance by analogy with animal

antibodies. The phytolectin role in plant protective reactions underthe different

host - pathogene interactions is indisputable. However, the alteration of

phytolectin composition and activity under virus infection and chemical

inductors of system ic acquired resistance was investigated rather poor.

The objective o f th is work is to study the interre lation between

hemagglutinin activity in healthy and virus infected plants and the effect o f

biochemical active agents (BAA) with immunoprotectorpropeties: arachidonic

acids, symbiont, alfa-tocopherol, synthetic coferment Q10 and composition

o f two last compounds.

Test-tube growed potato plants, cv Nevski 2 week old were treated by

the immunostimulators and when 2 day passed out they were infected by

TMV, potato viruses X-, M- and Y-. Phytohemagglutinin activity was

determined in 7 and 14 days after the onset o f vims infection. We find out an

absence of thestrict corralation between the antiviral and phytogemagglutinin

activities o f searching immunostimulators in model system potato plants

vitro -

phytoviruses. It should be assumed on the basis o f the obtained

results that the mechanism o f BAA immune action, probably, has no lectin

nature.

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