ЭлБиб

453 / 592

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
MICROPROPAGATIONOFDELPHINIUM UKOKENSE (SERG.)
L.V.Pronkina.
Botanical Garden ofA ltay State University, Barnaul, E-Mail:
biochem@biogeo.
Delphinium ukokense (Serg.) is localendemic species of Altay. It meets
in lim ited territory and has narrow-ecological amplitude. This species is
recommended fo r state protection. Because of difficult introduction and fo r
protection genofond o f this rare species it was attempt to decide this problem
by in vitro culture methods. We used 0,1% <Belosept-M> (20 m in.) and
0,1% HgCL2 (2 m in.)fo r sterilization of seeds. Only single seeds were
germ inated on Murashige and Skoog basal medium added w ith I mM 6-
benzylaminopurine (BA). Various concentration of BA (2-10 µM ) was studied
fo r m icropropagation. Coefficient o f propagation was 2-4 shoots per one
explant. Increase level of BA up to 20 mM resulted to higher yield of adventive
sh oo ts : 7-10 sh oo ts pe r one e xp lan t. Low c o n c e n tra tio n s o f 1-
naphthaleneacetic acid (1-3 µM) or indole-3-butyric acid (3-5 pM) stimulated
rooting.Morphology o f roots was dependenced from using auxins.
4S4
Научная электронная библиотека ЦНСХБ