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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

OPTIMIZATION OF CLONAL GRAPEVINEMICROPRAPAGATI ON

N.P.Doroshenko.

Russian Viticultural an dWine-makingResearch Institute named afterIa. Potapenko.

Meristem ic explants of small (0,17-0,25 mm) and extras- mall (0,075-

0,1 mm) sizes were used fo r healthening plants against phyloxera,

nematodes, fungi and bacterial pathogene infection. To increase their

regeneration activity the phase o f introduction was divided into two with,

alteration of hard and liguid culture medium, content o f 6-BAP at every phase

was defined and the possibility of low intensity EMI applying was investigated.

It was determined that influencing of EHR rays togetherw ith narrow stripped

lazers upon meristem, planted in hard culture medium, resulted in 2,5 folds

their regenerative ability. Ways o f optimization clonal micropropagation during

microculting

in vitro

plants were investigated: applying of EHR rays, vegetative

supplement o f fine-milled grapevine seeds, introducing o f 0,05 mg/l 6-BAP

into culture medium. Daily growth rate increases in 1,7-2,4 times under

EHR raysinfluencing, plant weight (mass) twicly, shoot length in 1,7, node

formation number 1,2-1,5 folds. Applying natural growth stimulators, o f fine

- m illed grapevine seeds into cultural medium, increases to 27,4% the

effectivness of clonal micropropagation. Introduction o f 0,5 mg/l 6 BAP into

culture medium increases microcutting more than twicly. Improving ways o f

plant adaptation permits to rise surviving ability of plants in the process of

laboratory adaptation up to 96,6-97,4%. The technology o f laying nursery

w ith healthied plant material of clonal micropropagation was developed fo r

stationary greenhouses as well as fo r covered ones and in tho opend soil.

Following this recommendation procides high surviving ability (80-90%), good

shoot development (117,6-184,1 cm) and maturing (109,1-138,7 cm) wich

0,5 cm in diametre, thats why in a year it is possible to lay nursary and next

year to begin preparing cuttings.

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