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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
MICROCLONAL PROPAGATION
OF
BETA VULGARIS
V .V.Snamenskaya, O.A.Podvigina
TheAll-Russian Research Institute o fSugar Beet & Sugar (VNIISS), Ramon, Voronezh
region
In connection with development of line heterosis breeding of sugar and
fodder beet, obtaining, storing and maintaining of valuable starting material
have become actual in the last decade. To use inbreeding for these purposes
is difficult because the quantity of seed formed with the help of self-pollination
is not enough, and due to a strong depression o f the lines obtained, or, in
most cases, to loss of all breeding material. To obtain, propagate and keep
the parent lines genetically identical for a long time, investigations on
m icroclonal propagation o f sugar beet under sterile conditions have been
started.
It is found, that induction o f sugar beet axillary buds development
in
vitro
is initiated by presence of combination of 6-BAP, kinetine, and gibberelline,
w ith 1:1:1 ratio and concentration o f 0.2-0.3mg/l, in a nutrient medium.
Positive regulatory effect o f the three growth substances is manifested in
formation o f 2-20 shoots per one explant and development o f 90.0-94.3%
normal plants.
Initiation o f axillary meristem proliferation and formation of 4-12 shoots
per one explant of fodder beet is achieved by reducing the quantity of 6-BAP
to 0.1mg/l, and adding 0.005mg/l o f NAA. Using the nutrient medium o f well-
balanced composition resulted in providing the maximum rate o f shoot
propagation w ithout their physiological alteration (shoot turning yellow,
crooked shoots, vitrification o f shoots), that allowed to propagate more than
700 sugar beet breeding lines, the survival rate being 88.3%, and more than
50 fodder beet breeding lines, the survival rate being almost 95%.
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