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H E A LT H Y P LA N T S A N D M IC R O P R O P A G A T IO N
PROPAGANATIONOFPEACH IN VITRO
N.M.Gutieva
Research institute o ffloriculture and subtropic crops. Sochi Fabriciusa street 2/28, E-
Mail:
Peach is the leading stone fruit crop Russia"s South. The aseptic culture
o f short tips is used for rapid propagation of virus-tested varieties.
Actively growing shoot tips o f Kollinz, Red Mountain & Veteran gathered
in spring.
They were treated in 0,02% mercurio chloride fo r 5 min & rinsed 3
tumes in autoclaved water.
Explants were cultured on Muroshiga & Skoog basal nutrient medium
suplimented with vitamins, casein hudrolysate, glycine, myo-inositol, sucrose,
agar & growth regulators. Addition of 1 m g /l of BA stimulated callus formation
while 0,1-0,5 mg/l o f BA was optimal fo r explant growth. Addition of IBA &
BA induced the formation of axiallary buds in explant.
Rooting was induced in 1,5-2 cm axiallary shoots. Explants o f two
varieties (Veteran & Kollinz) needed priminarily wetting for 12 hours in IBA
solution before planting onto growth regulator-free medium.
Explants o f other varieties formed roots after planting them onto he
medium with 1mg/l IBAwithout wetting.
Percentage of rooted explants differed from 30% (Veteran) to 60% (Red
Mountain).
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