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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

HATCHING ABILITY OFFREEEGGS OF

GLOBODERAROSTOCHIENSIS

UNDERAXENIC CULTURE OFPOTATO

N.Iddagoda and N.Illanganthilake.

Institute o f Fundamental Studies, Hantana Road, Kandy, Sri Lanka. E-mail:

The relative ability of potato root diffusate (PRD) from different genotypes

to induce hatch of free eggs of golden cyst nematode

(Globodera rostochiemis)

under" axenic culture conditions was examined. Murashige & Skoog basal

medium (MS) supplemented with 1.0 mg L-1indole-3-acetic acid (IAA) proved

to be successful in promoting good de-novo root formation (from the internode

stem segments o f 10 genotypes o f potato) and influencing inoculated free

eggs (FE) to hatch. Upon stimulation, free eggs o f the potato cyst nematode

(PCN) began to hatch erratically and second-stage juveniles (J2) emerged in

tiny wells located close to the growing roots. A relative hatching ability was

revealed among the genotypes according to the cumulative percentage hatch

(%CH) o f free eggs by the 32nd day o f incubation. The incubation period

(days) needed fo rthe emergence office highest number of J2(J2max) appeared

to be highly genotype-dependent. Hence, in addition to the prevalently used

mean percentage cumulative hatch of nematode eggs, a method is proposed

interconnecting this percentage with values of the emergence rate (ER) o f J2

[ER =(J2m a x /F E x 100 )/days] todemonstrate genotypic variation.

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