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H E A L T H Y P L A N T S A N D M IC R O PRO PAG A T ION

THE MICROPROPOGATION CONDITION INDUCESPECIFIC CULTURAL

PHENOTYPE

T.S.Gigolashvili, V.G.Reutski, O.I.Rodzkin

Institute ofexperimental botanyNationalAcademy o f Sciences o fBelarus.

Potato Research institute o fBelarusAgriculturalAcademy ofScience.

Abiotic factors of

in vitro

culture system induce unique culture phenotype

(CP). Plants with such phenotype posses an anatom ical and physiological

features differing from those of normal plants. The CP of

Solarium tuberosum

is characterised by tiny shoots, small leaves, reduced mechanical support

and conductive tissue, decreased epicuticular and a rticular wax, increased

percentage water content, non-functional stomata. Often this plants show

ex vitro

transplant stress and mortality. In this issue the micropropagated

plant survival increasing was actual.

Our interest was in cause of these phenotype.

Plant cultivation by commonly used method on culture medium (CM)

provides photomixotrophyc nutrition with heterotrophyc one being provided

by sucrose o f CM and auto trophyc - by pho tosyn thesis. To avoid

contamination of vessels, former are closed by lids. During the light period it

causes depletion o f C 0 2and autotrophic growth inhibition of plants. C 0 2

deficits induces appropriate adjustment o f leaves morphostructure, its

functional activity and the guard cell state as well.

W a te rsaturated atmosphere in the culture vessels and therefore a lack

o f w a te r potential gradient between surface o f plant and air leads to

transpiration inhibition and therefore limitation o f distant transport system.

The latest evoke absence of water loss regulation due to poorly developed

epicuticular wax layer as well as malfunctioning stomata’s and decreasing

osmotic potential.

Therefore, culture condition and adaptation to this specific complex of

abiotic factors

in vitro

induce appropriate adjustment and formed CP. After

the plants to be transferred into condition ex

vitro

this adjustment features

proved unrealisable. For increase the survival of explants demand eliminate

unfavourable factors during culturing

in vitro

the saturated atmosphere

hum idity and carbon dioxide deficits.

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