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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

MANIPULATION OF MEDIA COMPONENTS FOR

IN VITRO

MICROTUBERIZATION OF POTATO

(SOLANUM TUBEROSUML.)

Nissanka lddagoda

Ins t i t u t e o f Fundamen tal Studies, Hantana Road, Kandy, Sri Lanka. E-

Ma i l :

i dda@i f s . ac . lk

A fractional factorial experiment (FFE) was conducted to evaluate a

number of components in the growth medium that effect

in vitro

tuberization

o f potato (

Solanum tuberosum

). Sixteen treatments, composed of 4x4

symmetric factorial combination were imposed to select the optimal 4.0 mg

Г BAP x 80.0 g I' sucrose combination to serve as the basal solution fo r the

FFE. Cultures were incubated in dark fo r two months at 20°C. O f the II

media components (viz., X1- MS macro-nutrients,

X2 -MS micro-nutrients,

X 3 -FeNa.EDTA, X4 - glycine, X5 - nicotinic acid, X 6- pyridoxine-HCI, X7-

thiam ine-HC I, X8 - rnyo-inositol, X9- casein hydrolysate, X10- calcium

pantothenate, X 1 -biotin), only seven components (viz., X2, X3, X4, X7, X8, X9,

X 10) had effects on

in vitro

m icrotuber formation (viz., stolen length - SL,

number of tubers per explant - NT, microtuber fresli-weight - MFW, and cross-

sectional tuber area - CSTA). Among the effective factors, the factor

FeNa.EDTA, thiam ine-HC I and Calcium-pantothenate expressed their

influential contrary effects between cross-sectional tuber area and number

o f tubers per explant. W ith FeNa.EDTA, the contrary effects expressed

between stolen length and number of tubers, and by myo-inositol, between

stolen length and fresli-weiglit at tlie ir test range o f concentrations. Tilis

manipulation would be useful to determine the diversified effects of several

media components on

in vitro

microtuberization in a single, experiment that

may postulate for selecting the most suitalile composition of the tuberization

medium.

4 2 2

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