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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

ALTERATIONOFPHYTOHORMONESCOMPOSITIONINPOTATO

IN

VITRO

UNDERIMMUNOPROTECTORSIMPACTANDVIRUSINFECTION

G.A.Gerashchenkov,N.A .Rozhnova, S.Y.Gilyazetdinov

Department ofBiochemistry and Cytochemistry, UfaScientific Center, RussianAcademy

o fScience, 69, prospekt Oktyabrya, Ufa, 450054, Russia, E-Mail:

molgen@chembio.bashkiria.su

Being the participants of sygnal transduction processes, phytohormones

draw into a plant immunity response. Virus infection and immunostimulating

effect o f inductors o f system ic acquired resistance cause a phytohormone

com position changes in qua lity and quantity. These changes often

accompany a progress o f visible symptoms of diseases, hypersensitive

response and systemic acquired resistance. For example, active expression

o f PR-protein genes and their synthesis

de novo

can be regulated by

hormons. Thus, inhibition of virus reproduction in plants is mediated by

hormone effects. However, items of information on alteration o f hormone

balance in reply to virus infection and prelim inary elicitor effect are often

contradictory.

The objective o f the research was investigation o f dynam ics o f

phytohormone accumulation: abscisic acid (ABA), auxin (AX) and zeatin-

like cytokinin (ZCN) in test-tube grown potato, cv Nevski underthe impact of

b io chem ica l a c tive agents (BAA) w ith imm unop ro tecto r properties:

arachidonic acids, symbiont, alfa-tocopherol, synthetic coferm e n t Q10 and

phytoviruses: TMV and potato virus X.

It was revealed, that immune inducing effect of BAA was characterized

by quantitative alteration of phytohormone spectrum. The ABA and ZCN

compositions had the tendency to increase at virus infection and reached

maximum values as a rule only after a week infection period. In a control

(without immunization) infected plants demonstrated 1 .5 -3 times increases

o f ABA composition and essential lowering AX composition. It should be

noted that obviously expressed delay effect of this tendency in immune

plants was observed only in a week. It was demonstrated that BAA protection

effect in potato

in vitro

under virus infection has delayed character and

accompanied the alteration in phytohormone system.

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