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CELL CULTURE FOR PRODUCING PLANTS RESISTANT TO BIOTIC AND ABIOTIC
FACTORS ____ _____
ETHYLENEEXTRACTIONBYCULTURALWHEATCELLS
T.G.Karagezov, N.N.Lukanina, MG.Mamedova, J.A Aliev
Institute o fBotanyAcademy o fScienceAzerbayjanRepublic, 370073Baku,
Patamdarskoye shosse 40, E-Mail .
Under normal conditions (26°C) the depedence on the level and character
of ethylene syntesis determined by genetical determinated level of tolerance
in cultural strains from unripe embroyenes o f wheat was not established.
Under the temperature of 32°C fo r 2 hours followed by transfer to normal
conditions, the cellular strains of all genotypes, almost on the first hour of
changing of condition about 50% decreasing of the level o f ethylene synthesis
took place.The latter circumstances was differed on the level of changing fo r
different types. The increasing of ethylene synthesis ( maximum after 3-h o f
regeneration of control conditions) was odserved after2 hours. The magnitude
ofthese maximums did not depend on the level of tolerance and corresponded
to intensity differences of ethylene extraction by genotypes in the absence
o f stresses. In the following the decreasing of ethylene extraction was found
in all genotypes. Note that in non-tolerant species o f Diamant and mid-
tolerant Kazakhstanskaya-126, the decay take place until 7 hours while in
the case o f species with more level o f tolerance-Skala, Garakylchig-2,
Eritrospermum 841 at the begining o f 5-hours, the 2-nd peak o f ethylene
formation which is reached to maximum on 6-hours o f incubation was
found.This ethylene peak was specific to tolerant types and determined by
us as specific reaction to short-temperature effect.Growing cellular strains
at high temperature (32°C) for 1 month also result to the appearance of the
2nd peak o f ethylene formation in tolerant types.Cultivation under a high
temperature for3 months lead to equlibration in the level of ethylene production
in all genotypes. The formation o f the 1-st peak of ethylene is time constant
although the character of its dynamics is changeable.The 2nd peak ethylene
belonging to the tolerant types was also observed in adapted cellular strains
with a low level o f tolerancy-Diamant and Kazakhstanskaya-126.
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