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C E L L CU LTURE FO R PRODUCING PLANTS R E SISTA N T TO BIOTIC AND ABIOTIC

FACTORS

CELLSELECTION OFPOTATOTO STEROLINHIBITINGFUNGICIDE

PROPICONAZOL

K. A.Golovkin.*A.B.Burgutin.*A.N.Vorobiev, *R.G.Butenko,**U.T.Diakov.

Academy ofagricultural ofTurkmenistan, 744000, Ashgabad. Azadi 59;

*TimiryazevInstitute o fPlant Physiology 127276Moscow, Botanicheskaya 35;

**MoscowState University. BiologyDepartment, 117234Moscow, Vorobievy hory.

Using in selection system of triazol flingicides causes break of normal

sterol biosynthesis in plants by blocking of cylochrome P450-depent 14 a-

demethilation, In this work we used propiconazol us a seleclive agent which

inhibited clon formation of

S.tiberosum

cv.Tawa in 20 mkM concentration on

Murashige-Skoog agar medium. In selective conditions the treatment of cell

suspension by N-nitrozo-N-methilurea in I mM/h and 5 mM/h doses increased

the effect of the don formation. We yielded more than 800 clones in the

series of trials on different treatments of mutagen in 0-passage on selective

medium. There was selected 9 cell lines after 8 cultivation cycles on selective

medium. To verify the stability of resistance, the clones were cultivated 2

cycles

on non

selective medium. Then they were returned to selective

conditions again. The 5 from 9 cell lines maintained the capacity to grow in

se led ive condil ions. The analysis of dynamics o f sitosterol, stigmasterol

and campesterol content was carried out fo r callus tissue of resistant cell

lines and control ones. The level of campesterol content increased in more

than 28 times comparing to control one was observed in the presence o f

propiconazol in one cell line. After three non seledive passages the levels o f

sterol content were not different from control levels in this line.The level o f

sitosterol content o f the other cell lines increased in more than 50 times in

these conditions. Additional three non selective passages led to stabilisation

o f sterol content levels. In our opinion, the possible mode of resistance o f

the potato tissue culture to propiconazol is superproduction o f C 14 a -

demethilase.

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