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C E L L CU LTUR E FO R PRODUCING PLANTS R ESISTA N T TO BIOTIC AND ABIOTIC

_________

_____

______ FACTORS

TILLETIA

CARIES

INFLUENCEONTHEGROWTHOFTHEWHEAT

CALLUSCULTURESWITHDIFFERENTGENOMEORIGIN

I.V.Maksimov, R.M.Ganiev, O.B.Surina, R.F.Isaev, R.M.Khairullin

Department ofBiochemistry &Cytochemistry, Ufa Scientific Centre, RussianAcademy of

Sciences, Pr. Oktyabiya 69, Ufa, 450054, Russia. E-Mail:

molgen@chembio.bashkiria.su

The most effective donor of immunity of the cultured wheat to common

bunt is

Triticum timopheeviZhuk.,

which resistance is related with fungi

mycelium lysis.

We have obtained dual cultures of phytopathogen

Tilletia caries

Tu ll.

with callus from the mature germ of susceptible species

(T.durum

(AB

genome),

T.aestivum

(ABD genome)) and resistance species T. timopheevi

(AG genome) and shown successful growth and sporification of the pathogene

(Maksimov et al., 1997). In this connection we have decided to obtain the

same cultures of proposed donors of A, В and G genomes from diploid

species o f wheat and aegilops, those are resistant to the studied

phytopathogene in field conditions. (Isaev R.F., 1989).

It turned out, that infection of callus cultures by

Tilletia

teliospores leads

to essensial changes of morfo-physiological indices of callus. We have

obse rved fa s t accum ula tion of ca llus vo lum e in the expe rim en t.

Simultaneously with volume accumulation there are some separate embrionic

globules formation on dual cultures

T.urartu

(A genome). Thus such callus

became friable. On the other hand if on donors of genomes В and G

phytopathogene began to develop at once (10-20 days after infection), on

T.urartu

first symptoms of callus infection appeared only after the second

transplantation (30- th day after combained cultivation). The growth of dual

culture of

Tilletia caries

with

Ae.longissima

(B genome) and Ae. speltoides

(G genome) continued 60 days, and then came down and on terminal stadies

o f cultivation (90-100 days) no material growth was observed.There wasn't

any repression of growth of dual culture untill 100 days in the case of

T.urartu.

So we have shown the differents between degrees of phytopathogene

colonozation of callus cultures of diploid species of wheat and aegilops, and

the different reactions of proposed donors of genome A, В and G to the

phytopathogene infection.

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