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CELL CULTURE FOR PRODUCINO PLANTS RESISTANT TO BIOTIC AND ABIOTIC
___________________
FACTORS
THE RESPONSE OF IMMATUREEMBRYOS
OF
T.DURUM
AND
T.AEST IVUM
TO
VARYINGNICKEL CONCENTRATIONS
E.D.Nikitina
Altai Research Institute ofSoilManagment andPlantBreeding, Barnaul
Anthropological processes have caused the pollution o f large areas o f
land with heavy metals. To solve the problem integrated approach is required.
This w ill include the development of stress-resistant varieties. One method
o f doing this is cell selection.
To evaluate inter- and intraspecific polymorphism caused by nickel (Ni)
in immature embryos was used whole number of different varieties of bread
(b) and durum (d) wheat wiht known resistance: Novosibirskaja 67 (b),
Altaiskaja 50 (b), Altaiskaja Niva (d), Altaika (d) - intermedium resistant,
Chinese Spring (b) - low resistant. The selective system, that was used, the
medium developed by Linsmaierand Skoog (1963) containing different doses
o f Ni (0 ; 0.5; 1; 2; 3; 4; 6; 8; 9; 10 mg/l). The response of cell culture was
evaluated according to dry weight and the frequency of regeneration.
A non-linear relationship was established between the dry weight of
callus and the amount of Ni in the medium. Concentrations 1 ,4 ,6 mg/l gave
a stimulatary effect. The dry weight of callus of bread wheat was significantly
effected by genotypes, concentrations and their interactions. However only
the concentration of Ni was found to be significant in T. durum.
Morphogenic response to Ni is species-specific. Regenerat plants o f T.
durum were grown using Ni 10 mg/l. The bread wheat varieties have a lower
number o f regenerants (1.4%) in the medium containing 3 mg/l Ni. There
are varietal differences. So regeneration o f variety Altaiskaja 50 is totally
inhibited by Ni at 2 mg/l, whereas o f Chinese Spring it made up 50%.
Thus inter- and intraspecific polymorphism on regeneration ability was
observed among wheats tested to the concentrations of Ni. A non-linear
relationship between frequency o f regeneration and concentration of Ni was
established.
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