ЭлБиб

C E L L SE L E C T IO N

STABILIZATION OFSYNTHESISALKALO ID BERBERINE IN CELL

CLONES

THALICTRUMMINUS

E.A.Osipova, N.S.Chibulko, Z.B.Shamina

Timiryazev Institute o fplantPhysiologyRussianAcademy o fSciences, Botanicheskaya

Str., 35, Moscow, 127276, Russiafax: (095) 482-16-85, E-Mail:

The selection cell clones, producing biologically active substances is

very difficult to choose criteria. Using of selective systems enables to receive

resistant variants, it does not always correlated with synthesis secondary

metabolites. Visual markers can facilitate selection of productive clones. In

this respect

Thalictrum minus

is good object, as the quantity berberine,

correlates with color of cells and medium. To obtain stable cell lines to

many traits is complicated in connection w ith significant variation at

cubcultivation. Therefore it is important to know, when and how to estimate

clones in absence o f the selective factors.

For the decision o f this task primary selected on intensity o f color agar

medium seven clones were suspended and during 20 cubculture, were

analyzed the berberine content on the medium by spectrophotometry method.

During eight cubculture the berberine contents, as a rule, does not

exceed its in the initial strain and change from passage to passage (coefficient

o f a variation from 50 up to 90%, in the control - 30 %). Berberine content in

medium from selected clones was stabilized after 1,5 years (decrease of

coefficient of a variation). One part of clones observed precise decrease the

contents alkaloid, in others - increase.

As a result it was obtained two clones 124 and 233the berberine content

o f them were up to 420 and 440 mg/l, that higher the control in 2,5 times.

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