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C E L L S E L E C T IO N
POTATOCELLBREEDING
T.N.Oleynik
Institutef o r Potato Research, Nemeshaevo, Borodyanka district, Kiev region, 255740,
Ukraine, E-Mail:
Developing potato varieties resistant to diseases and pests by traditional
breeding methods is a long process. It depends on presence o f valuable
parental material, reliable methods o f assessing and selecting plants for
breeding, large amount o f work and material costs. Besides, genetic base
o f potato breeding requires regular widening.
In some cases cell technologies can supply and promote the breeding
process, offer genetic diversity at somatic cell level and make it possible to
screen genotypes having good economic characters. In the process of potato
ceil breeding for resistance to pathogens we used following selective agents:
1) complex of exoenzyms of Erwinia atroseptica, blackleg pathogen;
2) toxin of Corynebacterium sepedonicum, ring rot pathogen;
3) 1.3-1.6 glucans, Phytophthora infestans eliciters
Different selective agents require different experimental approaches and
interpretation o f obtained results. For example, the role of toxins in plant
diseases is not always clear.
Studying correlation o f resistance to pathogen in vitro and in vivo is a
necessary step. It makes possible to some degree to assess the expression
o f resistance character at cell level and further in tuber generations.
Lines of following varieties: Borodyansky rozovy, Vodogray, Zarevo,
Kobza, Lugovskoy, Nezabudka, Phytophthoroustoychivy and others with
wide resistance range have been obtained by the research. 10% o f the lines
have high resistance (8-9 points) which is stable in tuber generations; it
permits to involve them into reeding process.
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