ЭлБиб

SOMATIC HYBRIDIZATION

SOMATICHYBRIDIZATIONINSOLANUM

G.A.Yakovleva,T.V.Semanyuk,V.L.Dubinitch,O.V.Makhanko

Belarussian Research Institute forPotato Growing, Samokhvalovichi, Minsk reg.,

223013 Belarus

Wild species of potato possess various different types of resistance to

potato pests and diseases. The wild species often cannot be crossed

successfully with each other and with

Solarium tuberosum.

It is possible to

overcome sexual incompatibility by fusion o f somatic protoplasts.

In our investigation we used non-tuberous species

S.caripense

(resistant

to Colorado Potato Beetle (СРВ) and late blight) and interspecific hybrid

S.etuberosum

S.brevidens

(resistant to viruses) and also tuberous wild

species S.bulbocastanum (resistantto late blight),

S.polyadenium

(resistant

to insects and late blight) and chlorophyll deficient mutant of

S.tuberosum

(78563-76).

According to the preliminary data somatic hybrids were obtained from

fusion products of mesophyll protoplasts in 3 combinations: 1) S.polyadenium

(+) S.etuberosum x S.brevidens; 2) 78563-76 (+) S.bulbocastanum; 3) 78563-

76 (+) S.caripense (irradiation prior to fusion).

The single regenerant from combination 1 had intermediate phenotype

in morphological characters and in leaf peroxidase isozymes patterns. But

it was unable to form tubers in greenhouse. Twelve green regenerants of

combination 2 were obtained from ten separate calli. The S.bulbocastanum

protoplasts were unable to regenerate plants under the applied culture

conditions. The obtained plants were not like both parents in field performance

and were resistant to late blight after artificial plant inoculation by

Phytophthora

infestans.

The green shoots of combination 3 formed microtubers

in vitro

and showed different leaf peroxidase isoenzyme patterns compared to the

parents.

3 3 3

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