ЭлБиб

C EL L SE L E C T IO N

THE SCHEME OF SELECTION

IN VITRO

FOROBTAININGRICECLONES

RESISTANTTOPYRICULARIA ORYZAE CAV.

S.L.Belyanskaya,

Z.B.Shamina, L.A.Volkova, *A.A.Aver’yanov,

**L.M.Gayvoronskaya

Timiryazev Institute o f PlantPhysiologyRussianAcademy ofSciences, Botanicheskaya

Str., 35, Moscow, 127276, Russia,fax:(095)482-16-85, E-Mail:

*All-russian research institute o f phytopathology o fRussian agriculturalAcademy,

Bolshye Vyazemy, Moscow region, 143050 Russia, phone 592-9263,fax 8-2-334-09-02

**Russian University ofPeople Friendship, Mikluho-Maklay Str., 8/2, Moscow, 117198

Russia,fax 433-15-11

The rice resistance to

Pyricularia oryzae Cav.

consists of integrated

defence mechanisms. One of them is the inactivation o f fungus enzymes by

different inhibitors produced by plants. Most of inhibitors are phenols or

oxidized phenol forms. Accumulation o f phenols compounds and activation

o f oxidazing enzymes (e. g. peroxidase) correlated with plants resistance to

pathogens. We suggested that cells enrichment w ith triptophane and

phenilalanine, phenol precursors, should increase phenol concentration in

rice cells and increase their resistance to pathogen. Using toxic analog o f

these amyno-acids, p-fluoro-d-phenyl-alanine (PFPA) o r 5-methyl-dl-

tryptophan (MT), provides the possibility to obtain clones with high production

respective amino-acids.

In our experiment we used callus tissues o f two varieties: Zenith (Z) -

vertical resistant and Sha-tiao-tsao (S) which is sensitive to Pyricularia.

Rice resistant clones were obtained according to a common cell selection

scheme. A t eight selective subculture, seven PFPA-resistant clones and

three MT-resistant were obtained from Z-cultivar, and fifteen PFPA- and seven

MT- resistant clones for S-cultivar.

Peroxidase activity in selected clones was lower than in the initial cultivar

calluses, considerable increasing after treating callus with elicitor from

Pyricularia oryzae

and achieved the maximum level after five hours. Activity

did not change next two days.

Among the clones obtained from sensitive cultiva r S, clone No. 106

manifested high peroxidase activity (over 20 times higher than in initial

calluses).

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