ЭлБиб

SECONDARY PRODUCTS OF CULTURED PLANT CELLS

LAPPACONITINECONTENT INA LONG-TERMACONITUM

LEUCOSTOMUMCALLUSCULTURE

R.D.Valievaf,*F.N.Staruchin, A.G.Mardamshin

Department o fBiochemistry and Cytochemistry, UfaScientific Centre, Russian Academy

o fSciences, 450054, Ufa, Prospekt Oktyabrya, 69, Russia, E-M ail:

*Institute o fBiology, Ufa Scientific Centre, RussianAcademy o fSciences, 450054, Ufa,

Prospekt Oktyabrya, 69, Russia

Lappaconitine used fo r making an antiarrhythm ical preparation o f

allapinine can be obtained from Aconitum leucostomum. This work was

aimed at analyzing the content o f the given compound in the callus tissue o f

Aconitum leucostomum.

This callus tissue obtained from mature embryos had been cultivating

in the darkness, at the 23-24°C on the MS medium fo r 3 years. Since the

callus tissue was characterized by plentiful excretion o f polyphenols

subcultivation o f callus was carried out after every 20 days. During all the

period of

in vitro

cultivation the given culture was characterized by low speed

o f increase o f raw biomass. The content o f diterpenic alkaloid in the callus

tissue of Aconitum leucostomum was defined by the HPLC method. Received

data showed that the content o f the sought fo r product o f secondary

metabolism and its deacylated form on the basis o f its dry mass made

0,0002% and 0,0005% correspondingly. In the 11-months-old callus these

indices were 0,0004% and 0,0003%.

Low content o f lappaconitine in the callus tissue can show that it is

metabolizing in the devided cells. During the active vegetative growth o f

Aconitum leucostomum the content o f lappaconitine on the roots is also

badly reduced.

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