ЭлБиб

SECONDARY PRODUCTS OF CULTURED PLANT CELLS

INFLUENCEO FELICITORSONGINSENG STRAINRESEARCH

D.A.Zhuravleva, V.A.Galynkin, L.I.Slepyan

ChemicalPharmaceuticalAcademy, St. -Petersburg, Russia.

Higher plants cells culture acquires importance both fo r biotechnology

fundamental research and practical application in medicine, cosmetology

and food industry. Patent search testify that "old" medical plants begin to

application for new fixing with more complete uncover of their biological active

substances (BAS) synthesis biosynthetic possibilities.

On principle new ginseng cell lines have been obtained by IFR-5

(GCCCHP N-11) strain grow on nutrition substances with addition of elicitors

secreted from Betula pubescens Ehr. leaves, Quercus robur L. bark,

Glycirrhiza glabra L. root, Cetraria islandica Ach.. Influence of these elicitors

different concentration on growth and biosynthesis of primary and secondary

metabolites growth in initial strain has been studied in the process of the

cell line selection. The optimal technology o f the initial strain cultivation with

model elicitors has been worked out.

The obtained cell lines differ from the initial strain in morphology and

phytochem istry composition. Together with the triterpenoid glycosides

inherent to ginseng these cell lines contain BAS complex not inherent for

these plants which is contained in brought elicitors, such as flavanoid

glycosides, tannins, lichen acids, calendulosides, carbohydrates, etc.

This method enables to obtain cell lines with average 15-20% increased

contents of extractive substances and increased BAS spectrum. The obtained

cell lines biomass can serve as the raw material for the new medicine

substances with the active substances unique combination and the new

valuable pharmacological properties.

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