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M ETABOLISM O F PLANT C E L L S IN CULTURE
PHYTOHORMONES INFLUENCEONANTIOXIDANTENZYMES
METABOLISM IN RAUWOLFIA SERPENTINA CALLUS TISSUE
N.V.KIRILLOVA, I.A.ORECHOVA, V.P.KOMOV
St.Petersburg State ChemicalPharmaceuticalAcademy 197376St.Petersburg Prof.
PopovStr., l4 E-mail:
Phytohormones are the natural plant cell component regulating cellular
metabolism. Rate of metabolic processes in the tissues, including cell growth
and development, is depended on composition, correlation and activity of
the enzymes. Therefore investigation o f phytohormones influence on the
enzymes metabolism is necessary fo r comprehending o f molecular
mechanisms of the cell activity regulation in plant tissue. We studied kinetin
and naphtyl acetic acid (NAA) influence on superoxiddismutase (SOD),
catalase and peroxidase activity during the growth of Rauvolfia serpentina
tissue culture (industrial strain K-27). Maximal points of SOD catalytic activity
were observed at 15th and 35-40th day o f tissue growth. Catalase activity
curve had three maximal pikes at 15th, 35th and 65th day o f the cultured
cells growth. We observed also that highest peroxidase activity were reached
by 40th and 60th day of tissue growth. Determined phase fluctuation of the
enzymes activities were probably connected with mitotic activity changes of
studied heteroploid cultured cells. When phytohormones were added in
cultured medium we observed that SOD activity increased 25 per cent.
Moreover, when kinetin and NAA were present earlier registrated maximum
of SOD activity at 15th day of tissue growth was absent. Presence of
phytohormones was result in decreased catalase activity during callus tissue
growth. When kinetin was added in cultured medium, peroxidase activity
significantly increased, but presence of NAA didn’t cause activity change.
Besides that, we studied phytohormones influence on SOD, catalase and
peroxidase molecular heterogeneity and turnover.
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