ЭлБиб

PLANT GENETIC TRANSFORMATION. CELL SELECTIONAND SOMATIC HYBRIDIZATION

THEEFFECTOFGENENICTRANSFORMATIONONTOMEDICAGOVARIA

GENERATIVESPHEREANDSEEDPRODUCTION

*V.V.Mazin,M.N.Agafodorova,I.V.Komeeva

*All-RussianResearchInstituteofAgriculturalBiotechnology, Moscow,

All-RussianFodderResearchInstitute, Moscowregion.

Agrobacterium

transformation of

Medicago varia

by vectors containing

genes of phytohormone biosynthesis, tox, NPTII and rol called out important

modifications of generative sphere of transformed plants. It was observed

both increase and decrease of pollen and ovule fertility, of pollen grain and

ovule quantity. The pollen fertility was changed from 9.9 to 95.4% and ovule

fertility from 16.8 to 42.9%, quantity of pollen grains in anther from single to

full anther. There were genotype with increased pollen fertility among Ri- and

tt-plants. It was established, that transformed plants seed formation ability

varied in dependence on generative sphere and pollination type. The next

specialities of transformed plants fruitage were discovered:

-the ovule fertility was the main limiting factors during self-fertilization;

-the seed formation ability was preserved when the pollen and ovule

fertility were low.

We have supposition, that along whith seif-incompatibility and pollen

and ovule quantity and quality there is supplementary factor defining the

seed production of transformed alfalfa plants.

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