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PLANT GENETIC TRANSFORMATION,CELL SELECTION AND SOMATIC HYBRIDIZATION
THEOBTAININGOFGENETICTRANSFORMEDPLANTOF
MEDICAGO
VARIA
ANDSTUDINGOFITSMORPHOPHYSIOLOGICALAND
BIOCHEMICALPARTICULARFEATURES
V.V.Mazin*,M.N.Agafodorova, I.V.Korneeva.
*All-RossianResearchInstitute ofAgricultural Biotechnology, Moscow
All-Russian FodderResearch Institute, Moscow region
G enetic tran sfo rm ation was ca rry out by strain o f
Agrobacterium
tumefaciens
LGV3850/pGLTr4 containing NPT II gene and cytokinin synthesis
gene,
A.tumefaciens
A281/pGV941tt w ith m arker gene, phytohormone
biosynthesis gene and delta endotoxin gene and wild strain o f
A. rhizogenes
A 4 [pR iA 4] (Ivashuta et al., 1995) with using o f different recipient systems
(Mazin e tal., 1995). It was seen wide spectrum of change ability both structure
o f above-ground part and another signs. It was marked considerable changes
in the habitus o f transform ed plants, m iddle length of stoot between knots
and it's quantity, num ber of lateral stems, colour of flowers et a l. The grow
speed after cutting was decreased. it w as seen substantial change o f root
system m orphology (4 morphotypes) and root weight. It was m arked plants
with adventitious roots on the decumbent stems. Transformed plant handles
had different ability to form roots and had different degree o f root system
development. Alfalfa genetical transformation was provided substantial effect
on the male and fem ale generative spheres and seed production. It was
established both increase and decrease o f the content o f chlorophylls (a+b),
carotenoids, free proline and crude protein in comparison with control plant.
In O ctober opposite to march the content o f free proline in control plants
increased at 5 tim es, in transform ed plants a t 5-14. Methods and results of
alfalfa transform ation are discussed.
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