ЭлБиб

287 / 592

PLANT GENETIC TRANSFORMATION. CELL SELECTION AND SOMATIC HYBRIDIZATION
PECULIARITIESOFCALLUSSO-ANDMORPHOGENESISOFIPT
REGENERATEDTOBACCOPLANTS
R.V.Makarova,T.A.Borisova
Institute ofPlantPhysiology, RussianAcademy ofSciences, Botanicheskaya 35, Moscow,
127276RussiaE-Mail:
The capacities fo r regeneration, callus formation, and organogenesis
w ere studied in tobacco ( Nicotianatabacum L .) plants, both wild-type (control)
and transform ed w ith the active ipt gene o f agrobacterium . Control plants
w ere capable o f form ing calli only during 7 weeks, transgenic - up to 10
weeks. High frequencies o f callus formation were observed in control explants
when various concentrations o f 2,4-D (2 and 1 mg/l) and kinetin (0,2 and 0,5-
1 m g/l, respectively) were added to the m edium ; in transgenic explants -
only when 1 mg/l 2,4-D and 0,1 mg/l kinetin were added. W e observed
auxotrophic form ation o f the primary ipt callus tissue. The morphogenic
potential of ipt callus proliferated on hormone-free medium could be improved
by adding kinetin. Regenerated shoots form ed by ipt explants in the light
varied in their morphology: easily rooting, normally developing plantlets;
rosette plantlets lacking roots and num erous m icroshoots. In darkness
shootlike and nonviable abnormal structures were form ed. Morphological
traits o f ipt callus are probably conditioned by their specific hormonal system.
Transgenic plants have been recieved from prof. E.S.Piruzian (Institute
o f M olecular Genetics RAS, Moscow).
287
Научная электронная библиотека ЦНСХБ