ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

THE INFLUENCEOFANTIBIOTICSONPLANTCELLS

M.P.Mouminova, F.S.Moukhamedhanova, S.A.Djataev

Institute ofGenetics andExperimentalBiologyofPlantsASRUz, P.O. 97, Tashkent 700

000, Uzbekistan, E-Mail:

GCEB@SATO.GOV.UZ

The transformation of plant cells by

Agrobacterium

suppose the

application of antibiotics. Resistance to antibacterial preparation is used as

selective marker. The widely used marker sign at transformation is resistance

to kanamycin (Km). Antibiotics of sefalosporines serium so as karbenicillin,

klaforan and cefotaxcim are used for the growth inhibition. The influence of

Km and Karbenicillin (Kb) on

Gossypium hirsutum

tissue,

Phaseolus aureus

Rox

. and

Cucumis sativus

have been studied.

Tissue- and organospecificity of antibiotic action is marked. Thus, on

the explants of investigated Km as for the concentration is increased so

callusformation, shootformation and plant growth are inhibited. The Kb action

is species - specific. As for as the concentration of present antibiotic is

increased till 600 mkg/ml intensified callusogenesis on cotton explants is

observed. Above 700 mkg/ml the callus formation inhibition is marked.

Cultivation of

Phaseolus

apexes on the medium with karbenicillin 300 mkg/

ml stimulate shoot formation. At 500-1000 mkg/ml intensified callusogenesis,

risogenesis, but the absence of morphogenesis were observed. It was also

discovered that the increased antibiotic dose (1000 mkg/ml) intensify

rootformation at concentration 500 mkg/ml induce the embryogenesis of

cucumber.

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